Western Blot Utility Key Terminology

NameDescription
ExperimentA project that investigates the effects of various compounds and doses on substances to assess the viability of a compound for drug use. In this application, an experiment is represented by a folder on the file system, typically containing images.
ImageA digital scan of an optimally exposed blot stained with a specific antibody.
LaneRepresents a single case containing a unique set of independent variables, such as drug type, dose, and time factor.
Region of Interest (ROI)A specific rectangular area of pixels within an image and lane that represents the effectiveness of a case. Intensity calculations are performed based on this region.